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23 Sep 2011 Learn about CRISPR, Cre-lox through self-paced online JAX MiniCourses techniques using the CRISPR/Cas9 and Cre-lox technologies. 1 Jun 2016 Both CRISPR and Cre-LoxP are tools for directing modification to a specific DNA sequence within the organism. Learn More instead of just disrupting the original DNA, the CRISPR-Cas9 can be coupled with a fragment of 16 Nov 2017 The CRISPR-Cas9 system is a powerful genome editing technology that . (F) SpCas9 and tdTomato activations using Cre recombinase in Cre-recombinase system is one of the best-studied and most commonly used SSR in . To unite Cre-loxP and CRISPR/Cas9 system has been a promising 11 May 2016 With the advent of CRISPR/Cas9 as a mouse genome modification tool, we . Cg-Tg(Sox2-cre)1Amc/J (Sox2-Cre, stock 8454) mice were Manipulating the Mouse Embryo: a Laboratory Manual, Cold Spring Harbor 19 Dec 2017 Simplifying the combined use of CRISPR-Cas9 and Cre-loxP technologies for the efficient generation of targeted conditional gene knockouts in 19 Dec 2017 of specific genes are the Cre-LoxP system and the CRISPR-Cas9 system. CRISPR-Cas9 is used to knockout an essential gene, whose CRISPR/Cas9, Easi-CRISPR, ES Targeting. Constitutive Knockout. Point Mutation Knock-in. Short Tag Knock-in. Cre Recombinase Knock-in. Reporter Knock-in.29 Apr 2016 21 Aug 2018 The self-paced Introduction to CRISPR/Cas9 and Cre-lox Technologies certificate program includes two MiniCourses: Basics of CRISPR/Cas9