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No data analysis technique can make good data out of bad data (Howard Shapiro's Seventh Law). Flow Cytometry Workflow: Four Main Steps. Fixation. • KeepWhich Fluorophores are Useful for Flow Cytometry? Data Analysis. Gates and Regions . Propidium Iodide Staining of Cells for Cell Cycle Analysis . readers of today's immunology literature. Although some laboratories have readily adopted today's improved FACS data acqui- sition and analysis methods, D. Angie Swindell, M.Sc. Sarah Bartram, B.Sc. Principles. Data analysis. Protocols. We will briefly cover light scatter and fluorescence, basics of data analysis and commonly used cytometry applications, while highlighting important concepts on Output data in flow cytometry is discussed in Chapter 3, “Flow. Cytometry Statistical Analysis”. Once you understand your experiment, you are ready to conduct Data analysis consists of displaying the data from a list-mode file in a plot, then measuring the distribution of the events within the plots. As mentioned