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3-Hour Glucose Tolerance Test · You should eat your normal diet prior to the day of testing. · Do not eat, drink, smoke, or exercise for at least 8-12 hours Drink 10 ounces (entire bottle) of the glucose drink and note the time. You must drink all 10 ounces within a 5 minute period. After drinking the glucose you LABORATORY PATIENT COLLECTION INSTRUCTIONS. 2 hour Glucose Tolerance or. 2 hour Pregnant Glucose Tolerance or. Gestational Diabetes Screen. FASTING is NOT required for this test. • OBTAIN 1 bottle of 50 gram oral glucose tolerance beverage from the Clinic Lab staff. • DRINK the entire bottle (10 INSTRUCTIONS FOR GLUCOSE TEST. You may have nothing to eat or drink EXCEPT FOR WATER for 2 ¾ hours prior to your appointment at our office.One Hour Glucose Test Instructions · Drink all of the glucola beverage within a five minute period. · Arrive at the lab (with your lab) slip 40 minutes after you