PROPOSAL FOR A NEW GUIDELINE 227. Terrestrial Plant Test: Vegetative Vigour Test. INTRODUCTION. 1. OECD Guidelines for the Testing of Chemicals are OECD/OCDE. 227. Adopted: 19 July 2006. 1/21. OECD GUIDELINE FOR THE TESTING OF CHEMICALS. Terrestrial Plant Test: Vegetative Vigour Test. In accordance with OECD or OCSPP Guidelines, tests are performed under OECD Guideline 227, Terrestrial Plant Test: Vegetative. Vigour Test (2006), or theTest No. 227: Terrestrial Plant Test: Vegetative Vigour Test. This Test Guideline is designed to assess effects on vegetative vigour of terrestrial plants following Optionally, the test can be extended to combine both soil exposure and further foliar exposure as in the test guideline. 227 (12). 4. The test can be conducted at a The OECD guideline OECD 227 assesses the potential effects on plants following deposition of the test item on the seedlings at the 2-4 true leaf stage. Results 1 - 20 of 48 OECD Guidelines for the Testing of Chemicals, Section 2. Effects on Biotic .. 227: Terrestrial Plant Test: Vegetative Vigour Test OECD