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primary cultures such as isolating and disaggregating tissues. Although a separate tissue culture room Home made freezer stock is not. [[PDF] READ] Plant Tissue Culture: A Home-Based Guide (How to Practice Plant Tissue Culture on a Budget) by Edward Johnson. Mobi Download Plant Tissue Typically, the culture room for growth of plant tissue cultures should have a temperature between 15° and 30° C, with a temperature fluctuation of less than ±12/TeachersGuide/PlantBiotechnology/Documents/PlantTissueCulture.pdf Kitchen Culture Kits. Brining plant tissue culture to the home and classroom. Tissue culture involves the use of small pieces of plant tissue (explants) which are cultured in a nutrient medium under sterile conditions. Using the.